KCET · BIOLOGY · STD 12 - 9. Biotechnology: Principles and Processes

The enzymes which are absolutely necessary for recombinant DNA technology are

A restriction endonucleases and topoisomerases
B endonucleases and polymerases
C restriction endonucleases and ligases
D peptidases and ligases

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Correct Answer

(C) restriction endonucleases and ligases

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(C) restriction endonucleases and ligases,
The enzymes absolutely necessary for recombinant DNA technology are restriction endonucleases and ligases. Restriction endonucleases cut DNA at specific sequences, while ligases join DNA fragments together.
Topoisomerases: Relieve DNA supercoiling but aren't essential for recombination.
Polymerases: Involved in DNA replication but not essential for recombination.
Peptidases: Process proteins, not involved in DNA manipulation.

Apply the relevant identity from the chapter and substitute the values established in the previous step, keeping the original constraint in view.

Use the simplified expression to isolate the unknown, then plug the result back into the question setup to confirm the working is internally consistent.

Cross-check against a boundary or limiting case. Both the dimensional balance and the qualitative behaviour at the extreme should agree with the candidate answer.

Combine the steps above to identify the correct option. The remaining choices each fail at least one of the consistency, dimensional, or boundary checks.

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