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KCET · BIOLOGY · STD 12 - 9. Biotechnology: Principles and Processes

rop gene which codes for the proteins involved in the replication of the plasmid pBR322 in E. coli is located at restriction site of

  1. A Hind III
  2. B EcoRI
  3. C Puv II
  4. D BamHI
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Correct Answer

(C) Puv II

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Detailed explanation

(C) Puv II,
rop gene which codes for the proteins involved in the replication of the plasmid pBR322 in E.coli is located at restriction site of Puvll. The repressor of primer (rop) gene codes for rop protein that controls number of copies and prevents the replication of pBR322.
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