KCET · BIOLOGY · STD 12 - 9. Biotechnology: Principles and Processes

With respect to DNA fragmentation
Statement A: Gel electrophoresis and elution are two important processes.
Statement B: After staining with ethidium bromide it has to be exposed to UV light.

A Only A is correct.
B Both A and B are correct statements.
C Only B is correct.
D Only A is correct and B is not correct.

Verified Solution

Answer & Solution

Correct Answer

(B) Both A and B are correct statements.

Step-by-step Solution

Detailed explanation

(B) Both A and B are correct statements.,
Both A and B are correct. The isolated DNA fragments can be separated by gel electrophoresis which separates DNA fragments on the basis of charge and molecular weight. The separated DNA fragments can be visualised only after staining the DNA with ethidium bromide followed by exposure to UV radiation.

Apply the relevant identity from the chapter and substitute the values established in the previous step, keeping the original constraint in view.

Use the simplified expression to isolate the unknown, then plug the result back into the question setup to confirm the working is internally consistent.

Cross-check against a boundary or limiting case. Both the dimensional balance and the qualitative behaviour at the extreme should agree with the candidate answer.

Combine the steps above to identify the correct option. The remaining choices each fail at least one of the consistency, dimensional, or boundary checks.

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With respect to DNA fragmentationStatement A: Gel electrophoresis and elution are two important processes.Statement B: After staining with ethidium bromide it has to be exposed to UV light.

Step-by-step Solution

With respect to DNA fragmentation
Statement A: Gel electrophoresis and elution are two important processes.
Statement B: After staining with ethidium bromide it has to be exposed to UV light.