KCET · BIOLOGY · STD 12 - 9. Biotechnology: Principles and Processes
Palaeontologists unearthed a human skull during excavation. A small fragment of the scalp tissue was still attached to it. Only little DNA could be extracted from it. If the genes of the ancient man need to be analysed the best way of getting sufficient amount of DNA from this extract is
- A subjecting the DNA to gel electrophoresis
- B treating the DNA with restriction endonucleases
- C hybridising the DNA with a DNA probe
- D subjecting the DNA to polymerase chain reaction
Answer & Solution
Correct Answer
(D) subjecting the DNA to polymerase chain reaction
Step-by-step Solution
Detailed explanation
(D) subjecting the DNA to polymerase chain reaction,
To obtain s sufficient amount of DNA from a small fragment, polymerase chain reaction (PCR) is the best method. PCR amplifies the extracted DNA, allowing for a larger quantity to be analyzed. Gel electrophoresis separates DNA fragments. Restriction endonucleases cut DNA into smaller pieces. Hybridizing with a DNA probe is used to detect specific DNA probe is used to detect specific DNA sequences. These techniques are not useful for amplification of DNA.
To obtain s sufficient amount of DNA from a small fragment, polymerase chain reaction (PCR) is the best method. PCR amplifies the extracted DNA, allowing for a larger quantity to be analyzed. Gel electrophoresis separates DNA fragments. Restriction endonucleases cut DNA into smaller pieces. Hybridizing with a DNA probe is used to detect specific DNA probe is used to detect specific DNA sequences. These techniques are not useful for amplification of DNA.
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