KCET · BIOLOGY · STD 12 - 5. Molecular Basis of Inheritance
Some of the steps of DNA fingerprinting are given below. Identify the correct sequence from the options given.
A. Electrophoresis of DNA fragments
B. Hybridsation with DNA probe
C. Digestion of DNA by RENS
D. Autoradiography
E Blotting of DNA fragments to nitrocellulose membrane
- A C-A-B-E-D
- B C-A-E-B-D
- C A-E-C-B-D
- D A-C-E-D-B
Answer & Solution
Correct Answer
(B) C-A-E-B-D
Step-by-step Solution
Detailed explanation
(B) C-A-E-B-D,
The techniques has following steps:
(i) Isolation of DNA from sample cell.
(ii) If DNA is less in amount, it can be amplified using polymerase chain reaction (PCR).
(iii) Digestion of this DNA by restriction endonucleases or RENS(C).
(iv) These DNA fragments are electrophoresed in agarose gel. These fragments can be visualized using EtBr-UV system (A).
(v) Separated DNA fragments are transferred to nitrocellulose membrane using Southern Blotting (E).
(vi) Probing for VNTRS is done using labelled DNA probes (B).
(vii) The hybridized fragment can be detected by autoradiography (D).
The techniques has following steps:
(i) Isolation of DNA from sample cell.
(ii) If DNA is less in amount, it can be amplified using polymerase chain reaction (PCR).
(iii) Digestion of this DNA by restriction endonucleases or RENS(C).
(iv) These DNA fragments are electrophoresed in agarose gel. These fragments can be visualized using EtBr-UV system (A).
(v) Separated DNA fragments are transferred to nitrocellulose membrane using Southern Blotting (E).
(vi) Probing for VNTRS is done using labelled DNA probes (B).
(vii) The hybridized fragment can be detected by autoradiography (D).
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