KCET · BIOLOGY · STD 12 - 9. Biotechnology: Principles and Processes
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
A. Ability to multiply and express outside the host in a bioreactor.
B. A highly active promoter
C. A site at which replication can be initiated
D. One or more identifiable marker genes
E. One or more unique restriction sites.
- A A, C, D and E only
- B A, site at which replication can be initiated
- C One or more identifiable marker genes
- D BB, C and E only
Answer & Solution
Correct Answer
(C) One or more identifiable marker genes
Step-by-step Solution
Detailed explanation
(C) One or more identifiable marker genes,
Plasmids are extrachromosomal DNA present naturally in a bacterial cell. They are small double-stranded and closed circular DNA. They are used in recombinant DNA technology as cloning and expression vectors. For acting as a vehicle for the desired gene, they should be able to multiply inside the host cell thus need a replication origin site, should have marker genes and unique restriction sites.
Plasmids are extrachromosomal DNA present naturally in a bacterial cell. They are small double-stranded and closed circular DNA. They are used in recombinant DNA technology as cloning and expression vectors. For acting as a vehicle for the desired gene, they should be able to multiply inside the host cell thus need a replication origin site, should have marker genes and unique restriction sites.
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