KCET · BIOLOGY · STD 12 - 5. Molecular Basis of Inheritance
E.coli bacteria grew in \({ }^{15} NH _4 Cl\) medium for several generations are allowed to grow in \({ }^{14} NH _4 Cl\) medium. After 2 generations, the bacteria are isolated from the result of the density gradient of DNA is
- A only hybrid DNA
- B both hybrid and heavy DNA
- C both heavy and light DNA
- D both hybrid and light DNA
Answer & Solution
Correct Answer
(D) both hybrid and light DNA
Step-by-step Solution
Detailed explanation
(D) both hybrid and light DNA,
The DNA that was extracted from the culture one generation after transfer from 15N to 14N medium [that is after 20 minutes; E. coli divides in 20 minutes] had a hybrid or intermediate density. DNA extracted from the culture after another generation [that is after 40 minutes, II generation] was composed of equal amounts of this hybrid DNA and of 'light' DNA.
The DNA that was extracted from the culture one generation after transfer from 15N to 14N medium [that is after 20 minutes; E. coli divides in 20 minutes] had a hybrid or intermediate density. DNA extracted from the culture after another generation [that is after 40 minutes, II generation] was composed of equal amounts of this hybrid DNA and of 'light' DNA.
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