NEET · Biology · STD 12 - 9. biotechnology principlas and process

The following diagram showing restriction sites in \(E\). coli cloning vector pBR322. Find the role of ' \(X\) ' and ' \(Y\) genes:

A The gene ' \(X\) ' is responsible for controlling the copy number of the linked DNA and ' \(Y\) ' for protein involved in the replication of Plasmid.
B The gene ' \(X\) ' is for protein involved in replication of Plasmid and ' \(Y\) for resistance to antibiotics.
C Gene ' \(X\) ' is responsible for recognitions sites and ' \(Y\) ' is responsible for antibiotic resistance
D The gene ' \(X\) ' is responsible for resistance to antibiotics and ' \(Y\) ' for protein involved in the replication of Plasmid.

Verified Solution

Answer & Solution

Correct Answer

(A) The gene ' \(X\) ' is responsible for controlling the copy number of the linked DNA and ' \(Y\) ' for protein involved in the replication of Plasmid.

Step-by-step Solution

Detailed explanation

Correct answer is option (\(1\)), because ' \(X\) ' in the given diagram is ori while ' \(Y\) ' is rop.

Apply the relevant identity from the chapter and substitute the values established in the previous step, keeping the original constraint in view.

Use the simplified expression to isolate the unknown, then plug the result back into the question setup to confirm the working is internally consistent.

Cross-check against a boundary or limiting case. Both the dimensional balance and the qualitative behaviour at the extreme should agree with the candidate answer.

Combine the steps above to identify the correct option. The remaining choices each fail at least one of the consistency, dimensional, or boundary checks.

Same subject

Explore more questions on app

From NEET

Explore more questions on app