NEET · Biology · STD 12 - 9. biotechnology principlas and process

Select the correct statement from the following

A PCR is used for isolation and separation of gene of interest
B Gel electrophoresis is used for amplification of a \(DNA\) segment
C The polymerase enzyme joins the gene of interest and the vector \(DNA\)
D Restriction enzyme digestions are performed by incubating purified \(DNA\) molecules with the restriction enzymes of optimum conditions

Verified Solution

Answer & Solution

Correct Answer

(D) Restriction enzyme digestions are performed by incubating purified \(DNA\) molecules with the restriction enzymes of optimum conditions

Step-by-step Solution

Detailed explanation

Option (4) is the correct answer. \(PCR\) is used for amplification of \(DNA\). Gel electrophoresis is used for separation of digested \(DNA\) fragments. Ligase enzyme joins the gene of interest and vector \(DNA\).

Apply the relevant identity from the chapter and substitute the values established in the previous step, keeping the original constraint in view.

Use the simplified expression to isolate the unknown, then plug the result back into the question setup to confirm the working is internally consistent.

Cross-check against a boundary or limiting case. Both the dimensional balance and the qualitative behaviour at the extreme should agree with the candidate answer.

Combine the steps above to identify the correct option. The remaining choices each fail at least one of the consistency, dimensional, or boundary checks.

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\((c)\) Scapula	\((iii)\) Clavicle
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\((a)\quad(b) \quad(c) \quad(d)\)

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