NEET · Biology · STD 12 - 9. biotechnology principlas and process

In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?

A Using ampicillin & tetracycline containing medium plate.
B Blue color colonies will be selected.
C White color colonies will be selected.
D Blue color colonies grown on ampicillin plates can be selected.

Verified Solution

Answer & Solution

Correct Answer

(C) White color colonies will be selected.

Step-by-step Solution

Detailed explanation

The correct answer is that white-colored colonies will be selected.
Since an alien piece of DNA is being inserted at EcoRI site, the gene \(\beta\)-galactosidase present here will undergo insertional inactivation.

This gene is responsible for producing blue-colored colonies, but since it has been insertionally inactivated, white colored colonies will be produced.

Ampicillin and tetracycline resistance genes present in the given DNA will remain intact. Thus, the given DNA will show amp \({ }^R\) and tet \({ }^R\).

Apply the relevant identity from the chapter and substitute the values established in the previous step, keeping the original constraint in view.

Use the simplified expression to isolate the unknown, then plug the result back into the question setup to confirm the working is internally consistent.

Cross-check against a boundary or limiting case. Both the dimensional balance and the qualitative behaviour at the extreme should agree with the candidate answer.

Combine the steps above to identify the correct option. The remaining choices each fail at least one of the consistency, dimensional, or boundary checks.

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List \(I\)	List \(II\)
\(A\). Robert May	\(1\). Species-Area relationship
\(B\). Alexander von Humboldt	\(II\). Long term ecosystem experiment using out door plots
\(C\). Paul Ehrlich	\(III\). Global species diversity at about \(7\) million
\(D\). David Tilman	\(IV\). Rivet popper hypothesis

Choose the correct answer from the options given below:

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\((c)\) Ascending limb of Henle's loop	\((iii)\) Conditional reabsorption of sodium ion and water
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