NEET · Biology · STD 12 - 5. molecular basis of inheritance

Arrange the following steps of DNA fingerprinting in a correct sequence.
A. Isolation of DNA and its digestion by restriction endonucleases.
B. Hybridisation using a labelled VNTR probe.
C. Transferring of separated DNA fragments to synthetic membranes.
D. Detection of hybridised DNA fragments by autoradiography.
E. Separation of DNA fragments by electrophoresis.
Choose the correct answer from the options given below :

A A, B, D, C, E
B A, D, B, E, C
C A, E, C, B, D
D A, E, B, C, D

Verified Solution

Answer & Solution

Correct Answer

(C) A, E, C, B, D

Step-by-step Solution

Detailed explanation

(C) A, E, C, B, D
The technique of DNA fingerprinting involves the following steps in sequence:
(A) Isolation of DNA and its digestion by restriction endonucleases (A).
(B) Separation of the digested DNA fragments by gel electrophoresis (E).
(C) Transferring (blotting) of the separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon (C).
(D) Hybridisation using a radiolabelled VNTR probe (B).
5. Detection of the hybridised DNA fragments by autoradiography (D).
The correct sequence is A, E, C, B, D.

Apply the relevant identity from the chapter and substitute the values established in the previous step, keeping the original constraint in view.

Use the simplified expression to isolate the unknown, then plug the result back into the question setup to confirm the working is internally consistent.

Cross-check against a boundary or limiting case. Both the dimensional balance and the qualitative behaviour at the extreme should agree with the candidate answer.

Combine the steps above to identify the correct option. The remaining choices each fail at least one of the consistency, dimensional, or boundary checks.

Same subject

Match List \(I\) with List \(II\)

List \(I\)	List \(II\)
\(A.\) Pons	\(I.\) Provides additional space for Neurons, regulates posture and balance.
\(B.\) Hypothalamus	\(II.\) Controls respiration and gastric secretions.
\(C.\) Medulla	\(III.\) Connects different regions of the brain.
\(D.\) Cerebellum	\(IV.\) Neuro secretory cells

Choose the correct answer from the options given below :

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