KCET · BIOLOGY · STD 12 - 9. Biotechnology: Principles and Processes
Which one of the following statements is wrong with respect to separation of DNA fragments on gel electrophoresis?
- A The DNA fragments move towards anode under electric field through the matrix.
- B The commonly used matrix is agarose gel.
- C The DNA fragments resolve according to their size.
- D The smaller DNA fragments separate first.
Answer & Solution
Correct Answer
(D) The smaller DNA fragments separate first.
Step-by-step Solution
Detailed explanation
(D) The smaller DNA fragments separate first.,
Gel electrophoresis is a technique that uses differences in electrical charges to separate the molecules in a mixture. Since DN molecules have negative charges, they migrate towards the positive electrode (anode) when placed in an electric field. This rate of migration depends on size. The smaller the DNA molecule, faster is the migration. So, the DNA band that is the farthest the well where it was loaded, is the smallest in size.
Gel electrophoresis is a technique that uses differences in electrical charges to separate the molecules in a mixture. Since DN molecules have negative charges, they migrate towards the positive electrode (anode) when placed in an electric field. This rate of migration depends on size. The smaller the DNA molecule, faster is the migration. So, the DNA band that is the farthest the well where it was loaded, is the smallest in size.
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