KCET · BIOLOGY · STD 12 - 9. Biotechnology: Principles and Processes
Identify the labels M and N in the following agarose gel electrophoresis representation.
- A M-Smallest DNA bands, N - Largest DNA bands
- B M Digested DNA bands, N- Undigested DNA bands
- C M-Hybridised DNA bands, N - Unhybridised DNA
- D M-Largest DNA bands, N - Smallest DNA bands
Answer & Solution
Correct Answer
(D) M-Largest DNA bands, N - Smallest DNA bands
Step-by-step Solution
Detailed explanation
(D) M-Largest DNA bands, N - Smallest DNA bands,
The cutting of DNA by restriction endonucleases results in the fragments of DNA. Since DNA fragments are negatively charged molecules they can be separated by forcing them to move towards the anode under an electric field through a medium/matrix. The DNA fragments separate (resolve) according to their size through sieving effect provide by the agarose gel. Hence, the smaller the fragment size, the farther it moves. Thus, M is the largest band and N is the smallest band.
The cutting of DNA by restriction endonucleases results in the fragments of DNA. Since DNA fragments are negatively charged molecules they can be separated by forcing them to move towards the anode under an electric field through a medium/matrix. The DNA fragments separate (resolve) according to their size through sieving effect provide by the agarose gel. Hence, the smaller the fragment size, the farther it moves. Thus, M is the largest band and N is the smallest band.
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