KCET · BIOLOGY · STD 12 - 9. Biotechnology: Principles and Processes

EcoRI cleaves the DNA strands to produce

A blunt ends
B sticky ends
C satellite ends
D ori replication end

Verified Solution

Answer & Solution

Correct Answer

(B) sticky ends

Step-by-step Solution

Detailed explanation

(B) sticky ends,
EcoRI is a restriction enzyme isolated from the bacterium Escherichia coli. It cuts DNA at the palindromic sequence 5'-GAATTC'3', specifically between the G and A on each strand, producing sticky ends with 5' overhangs.

Apply the relevant identity from the chapter and substitute the values established in the previous step, keeping the original constraint in view.

Use the simplified expression to isolate the unknown, then plug the result back into the question setup to confirm the working is internally consistent.

Cross-check against a boundary or limiting case. Both the dimensional balance and the qualitative behaviour at the extreme should agree with the candidate answer.

Combine the steps above to identify the correct option. The remaining choices each fail at least one of the consistency, dimensional, or boundary checks.

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