Which of the following features are required to facilitate cloning?
(A) Presence of origin of replication (Ori) in the cloning vector
(B) Presence of a selectable marker in the cloning vector
(C) Presence of a single recognition site (preferably) for restriction enzyme in the cloning vector
(D) Presence of more than two promoter regions in the cloning vector
Choose the correct answer from the options given below:

Apply the relevant identity from the chapter and substitute the values established in the previous step, keeping the original constraint in view.

Use the simplified expression to isolate the unknown, then plug the result back into the question setup to confirm the working is internally consistent.

Cross-check against a boundary or limiting case. Both the dimensional balance and the qualitative behaviour at the extreme should agree with the candidate answer.

Combine the steps above to identify the correct option. The remaining choices each fail at least one of the consistency, dimensional, or boundary checks.