We use microbes and microbially derived products almost every day. Bacteria called lactic acid bacteria (LAB) grow in milk to convert it into curd. The dough, which is used to make bread, is fermented by yeast called Saccharomyces cerevisiae. Microbes are used to produce industrial products like lactic acid, acetic acid and alcohol, which are used in a variety of processes in the industry. However, certain bacteria, which grow anaerobically on cellulosic material, produce large amount of methane along with CO2 and H2. These bacteria are collectively called methanogens, and one such common bacterium is Methanobacterium. These bacteria are commonly found in the anaerobic sludge during sewage treatment. Microbes are also used for commercial and industrial production of certain chemicals like organic acids, alcohols and enzymes.
Examples of acid producers are Aspergillus niger (a fungus) of citric acid. You are familiar with the commonly used antibiotic Penicillin. Do you know that Penicillin was the first antibiotic to be discovered, and it was a chance discovery? Statins produced by the yeast Monascus purpureus have been commercialised as blood-cholesterol lowering agents. It acts by competitively inhibiting the enzyme responsible for synthesis of cholesterol.
Which one of the following statements is not true with respect to microbes?

1. A Wine is produced by fermentation without distillation.
2. B Microbes can also be used to kill harmful pests, a process called as biocontrol.
3. C The puffed up appearance of dough is due to the production of CO gas by bacteria.
4. D Methanogens are anaerobic bacteria.