The correct sequence of steps to obtain gene of interest from pool of DNA is:
(A) Cutting of DNA using restriction endonuclease.
(B) Viewing the ethidium bromide stained gel under UV light.
(C) Loading of DNA in wells.
(D) Cutting and extraction of DNA piece from gel.
(E) Separation of DNA fragments according to their molecular size.
Choose the correct answer from the options given below:

Choose the correct option, which explains the term alleles.

Match List-I with List-II

List-I (Characteristics)	List-II (Examples)
(A) Symbiotic association with roots of leguminous plants	(I) Glomus
(B) Fixes atmospheric nitrogen while free living in the soil	(II) Rhizobium
(C) Symbiotic association of fungi and plants	(III) Anabaena
(D) Cyanobacteria which can fix atmospheric nitrogen	(IV) Azotobacte

Choose the correct answer from the options given below:

Read the passage carefully and give the answers to the question :
India was amongst the first countries in the world to initiate action plans and programmes at a national level to attain total reproductive health as a social goal.
These programmes called 'family planning' were initiated in 1951 and were periodically assessed over the past decades. Improved programmes covering wider reproduction-related areas are currently in operation. Creating awareness among the people about various reproduction-related aspects and providing facilities and support for building up a reproductively healthy society are the major tasks under these programmes.
Match Column I with Column II

Column I	Column II
(A) ZIFT	(I) Sperm injected into ovum
(B) ICSI	(II) Embryo in uterus
(C) IUI	(III) Embryo in fallopian tube
(D) IUT	(IV) Semen in uterus

Choose the correct answer from the options given below

Read the following paragraph and answer the given questions:
DRUGS AND ALCOHOL ABUSE
The drugs, commonly abused are opioids, cannabinoids, and coca alkaloids. Opioids are the drugs, which bind to specific opioid receptors present in our central nervous system and gastrointestinal tract. Heroin, commonly called smack is chemically diacetylmorphine which is a white, odourless, bitter crystalline compound. Cannabinoids are a group of chemicals, which interact with cannabinoid receptors present principally in the brain. Natural cannabinoids are obtained from the inflorescences of the plant Cannabis sativa. The flower tops, leaves and the resin of cannabis plant are used in various combinations to produce marijuana, hashish, charas and ganja. Generally taken by inhalation and oral ingestion, these are known for their effects on cardiovascular system of the body. It interferes with the transport of the neuro-transmitter dopamine. Cocaine, commonly called coke or crack is usually snorted. It has a potent stimulating action on central nervous system, producing a sense of euphoria and increased energy. Excessive dosage of cocaine causes hallucinations. Other well-known plants with hallucinogenic properties are Atropa belladona and Datura. These days cannabinoids are also being abused by some sportspersons. Drugs like barbiturates, amphetamines, benzodiazepines, and other similar drugs, that are normally used as medicines to help patients cope with mental illnesses like depression and insomnia, are often abused. Morphine is a very effective sedative and painkiller, and is very useful in patients who have undergone surgery. Several plants, fruits and seeds having hallucinogenic properties have been used for hundreds of years in folk-medicine, religious ceremonies and rituals all over the globe.
Which is used as an effective sedative and painkiller and is useful to patients who have undergone surgery?

In an ecosystem

Completion of the first meiotic division of the primary oocytes takes place -

The work function of a photosensitive material is 4.0 eV. The longest wavelength of light that can cause electron emission from the material is (approximately)

Select the correct statement.

A wheel with 10 metallic spokes each of length 1.0 m long is rotated with a speed of 240 rev/min in a plane normal to magnetic field of 0.4 G at the place, the induced emf between the axle and the rim of the wheel is: Given that 1 G = \(10^{-4}\) T.

Which species is involved in chlorination of benzene using \(Cl_{2}\) in presence of \(AlCl_{3}\)?

Match List I with List II

LIST I	LIST II
A. Range of \(\operatorname{cosec}^{-1} x\)	I. \((0, \pi)\)
B. Range of \(\tan ^{-1} x\)	II. \([0, \pi]-\left\{\frac{\pi}{2}\right\}\)
C. Range of \(\sec ^{-1} x\)	III. \(\left[-\frac{\pi}{2}, \frac{\pi}{2}\right]-\{0\}\)
D. Range of \(\cot ^{-1} x\)	IV. \(\left(-\frac{\pi}{2}, \frac{\pi}{2}\right)\)

Which of the following is not true with reference to evaporation of liquid?