CUET · BIOLOGY · PYQ PAPER 2025
Read the passage carefully to answer the questions given below
Some strains of Bacillus thuringiensis produce proteins that kill certain insects such as lepidopterans (tobacco budworm, armyworm), coleopterans (beetles) and dipterans (flies, mosquitoes). In Bacillus thuringiensis the toxin is coded by a gene crylAc named cry. There are a number of them, for example, the proteins encoded by the genes crylAc and cryllAb control the cotton bollworms, that of crylAb controls corn borer. Using conventional methods of diagnosis (serum and urine analysis, etc.) early detection is not possible. Recombinant DNA technology, Polymerase Chain Reaction (PCR) and Enzyme Linked Immuno-sorbent Assay (ELISA) are some of the techniques that serve the purpose of early diagnosis. The first clinical gene therapy was given in 1990 to a 4-year old girl with adenosine deaminase (ADA) deficiency. This enzyme is crucial for the immune system to function. The disorder is caused due to the deletion of the gene for adenosine deaminase. RNAi takes place in all eukaryotic organisms as a method of cellular defense. This method involves silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA.
RNAi involves involves silencing of a specific mRNA due to a complementary__________ molecule that binds to and prevents translation of the mRNA.
- A dsRNA
- B ssRNA
- C dsDNA
- D ssDNA
Answer & Solution
Correct Answer
(A) dsRNA
Step-by-step Solution
Detailed explanation
dsRNA
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In the year 1963 the two enzymes responsible for restricting the growth of bacteriophage in E. coli were isolated. The first restriction endonuclease - Hind II, whose functioning depended on a specific DNA nucleotide sequence was characterized five years later. It was found that Hind II always cut DNA molecules at a particular point by recognizing a specific sequence of six base pairs. This specific base sequence is known as the recognition sequence for Hind II. Besides Hind II, today we know more than 900 restriction enzymes that have been isolated from over 230 strains of bacteria, each of which recognize different recognition sequences.
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Some strains of `Bacillus thuringiensis` produce proteins that kill certain insects such as lepidopterans (tobacco budworm, armyworm), coleopterans (beetles) and dipterans (flies, mosquitoes). In `Bacillus thuringiensis` the toxin is coded by a gene `crylAc` named cry. There are a number of them, for example, the proteins encoded by the genes `crylAc` and `cryllAb` control the cotton bollworms, that of `crylAb` controls corn borer. Using conventional methods of diagnosis (serum and urine analysis, etc.) early detection is not possible. Recombinant DNA technology, Polymerase Chain Reaction (PCR) and Enzyme Linked Immuno-sorbent Assay (ELISA) are some of the techniques that serve the purpose of early diagnosis. The first clinical gene therapy was given in 1990 to a 4-year old girl with adenosine deaminase (ADA) deficiency. This enzyme is crucial for the immune system to function. The disorder is caused due to the deletion of the gene for adenosine deaminase. `RNAi` takes place in all eukaryotic organisms as a method of cellular defense. This method involves silencing of a specific `mRNA` due to a complementary `dsRNA` molecule that binds to and prevents translation of the `mRNA`.
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