Read the passage carefully and answer the given questions.
Recombinant DNA Technology Asexual reproduction preserves the genetic information, while sexual reproduction permits variation. Traditional hybridization procedures used in plants and animals breeding very often lead to inclusion and multiplication of undesirable genes along with the desired genes. The techniques of genetic engineering, which include creation of recombinant DNA, use of gene cloning and gene transfer, overcome this limitation and allow us to isolate and introduce only one or a set of desirable genes without introducing genes into the target organism.
Which one of the following options is correct for working of restriction enzymes?

1. A A tool used to insert DNA into the cells
2. B Enzyme used to join DNA fragments
3. C Used as vector
4. D Cuts DNA at specific sequence