Read the following passage carefully and answer the given questions
The separated DNA fragments can be visualized only after staining the DNA with ethidium bromide followed by exposure to UV radiation. Bright orange colored bands of DNA in ethidium bromide stained gel exposed to UV light can be seen. The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution. The DNA fragments purified in this way are used in constructing recombinant DNA by joining them with a cloning vector.
The DNA fragments are separated by-

Arrange the following events in correct sequence.
A. Formation of zygote
B. Formation of blastocyst
C. Implantation
D. Formation of morula
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Which one of the following approaches does not belong to ex situ conservation strategy?

Read the following passage carefully and answer the given questions.
The B-lymphocytes produce an army of proteins in response to pathogens. Each antibody molecule has four peptide chains, two small called light chains and two longer called heavy chains. Virus-infected cells secrete proteins called interferons which protect non-infected cells from further viral infection. Symptoms of allergic reactions include sneezing, watery eyes, running nose and difficulty in breathing. The use of drugs like anti-histamine, adrenalin and steroids quickly reduce the symptoms of allergy.
Lymphoid organs are the organs where origin and/or maturation and proliferation of lymphocytes occur. The primary lymphoid organs are bone marrow and thymus. After maturation the lymphocytes migrate to secondary lymphoid organs like spleen, lymph nodes, tonsils, Peyer's patches of small intestine and appendix. Heroin commonly called smack is chemically diacetylmorphine which is a white, odourless, bitter crystalline compound. This is obtained by acetylation of morphine, which is extracted from the latex of poppy plant Papaver somniferum.
Which one of the following organs is not a secondary lymphoid organ in humans?

Match List I with List II

List - I (Group)	List - II (Organism)
(A) Lepidoptera	(I) Beetles
(B) Bacteria	(II) Armyworm
(C) Diptera	(III) Bacillus
(D) Coleoptera	(IV) Mosquitoes

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Which of the following enzyme is used during isolation of DNA from fungal cells?

Match List-l with List-II:

List-I (Interspecies Relationships)	List-II (Features)
(A) Commensalism	(I) One species is benefitted at the expense of the other
(B) Mutualism	(II) One species is harmed and the other is unaffected
(C) Amensalism	(III) Both the species are benefitted
(D) Parasitism	(IV) One species benefits and other remains unaffected

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In a series LCR circuit, \( C = 80 \, \mu\text{F} \), \( L = 5.0 \, \text{H} \) and \( R = 40 \, \Omega \) are connected to a variable frequency source of 240 V. The angular frequency of the source which drives the circuit at resonance will be:

Arrange the following steps \(( A , B , C\) and D \()\) in proper sequence to determine the index correction in the given arrangement.

(A) The difference between the two gives the correction to be applied to the observed distance.
(B) The positions of the uprights are read on the scale. Their difference gives the observed distance between tip of the pin and the pole of the mirror.
(C) A thin straight index needle OP is placed so that its one end O touches the tip of the pin and the other end P touches the pole of the mirror.
(D) Length of the needle OP is measured by placing it on the scale which is the actual distance between the points in question.
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The degradation of humus by microbes to release inorganic nutrients is known as-

Minimum boiling azeotropes show __________ deviation from Raoult's Law at a specific composition.

The unit of conductivity (K) is:

Which one of the following hormones is not secreted by the placenta?