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CUET · BIOLOGY · PYQ PAPER 2025

Read the following passage carefully and answer the given questions
The separated DNA fragments can be visualized only after staining the DNA with ethidium bromide followed by exposure to UV radiation. Bright orange colored bands of DNA in ethidium bromide stained gel exposed to UV light can be seen. The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution. The DNA fragments purified in this way are used in constructing recombinant DNA by joining them with a cloning vector.
The DNA band appears orange due to -

  1. A Staining with acetocarmine and exposed to with uv light
  2. B Staining with ethidium bromide and exposed to uv light
  3. C Staining with ethidium bromide and exposed to infra red light
  4. D Staining with acetocarmine and exposed to infra-red light
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Correct Answer

(B) Staining with ethidium bromide and exposed to uv light

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Staining with ethidium bromide and exposed to uv light
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