Read the following paragraph carefully and answer the given questions.
In the year 1963 the two enzymes responsible for restricting the growth of bacteriophage in E. coli were isolated. The first restriction endonuclease - Hind II, whose functioning depended on a specific DNA nucleotide sequence was characterized five years later. It was found that Hind II always cut DNA molecules at a particular point by recognizing a specific sequence of six base pairs. This specific base sequence is known as the recognition sequence for Hind II. Besides Hind II, today we know more than 900 restriction enzymes that have been isolated from over 230 strains of bacteria, each of which recognize different recognition sequences.
Restriction enzymes are also known as

Read the following passage carefully and answer the given questions.
During anaerobic sludge digestion, bacteria produce a mixture of gases such as methane, hydrogen sulphide and carbon dioxide. These gases form biogas and can be used as source of energy as it is inflammable. The bioactive molecule, cyclosporin A, that is used as an immunosuppressive agent in organ-transplant patients, is produced by the fungus Trichoderma polysporum. You must have noticed that bottled fruit juices bought from the market are clearer as compared to those made at home. This is because the bottled juices are clarified by the use of pectinases and proteases. Statins produced by the yeast Monascus purpureus have been commercialised as blood-cholesterol lowering agents. It acts by competitively inhibiting the enzyme responsible for synthesis of cholesterol. The BOD test measures the rate of uptake of oxygen by micro-organisms in a sample of water and thus, indirectly, BOD is a measure of the organic matter present in the water.
Cyclosporin A, that is used as an immunosuppressive agent in organ-transplant patients, is produced by -

The enzyme that can recognise the following palindromic sequence
'5-GAATTC-3'
3'-CTTAAG-5' is _________ :

Failure of chromatids to segregate during cell division cycle results in :

Agrobacterium tumifaciens, is able to deliver a piece of DNA known as ______ to transform normal plant cell into a tumor cell.

Which one of the following diagnostic techniques is based on the principle of antigen-antibody interaction?

Match List I with List II

List - I	List - II
(A) The cells which provide nutrition to the germ cells	(I) Interstitial cells
(B) The organ which helps in the lubrication of penis	(II) Bulbourethr al gland
(C) The pouch which helps to maintain testes temperature 2-2.5°C lower than normal internal body temperature	(III) Sertoli cells
(D) The cells which synthesise and secrete androgens	(IV) Scrotum

Choose the correct answer from the options given below:

Photoelectric current depends on
(A) the intensity of incident light
(B) the potential difference applied between the two electrodes
(C) the nature of the emitter material
(D) the conductivity of the emitter material
Choose the correct answer from the options given below:

2.0 g of benzoic acid dissolved in 25 g of benzene shows a depression in freezing point of 1.62 K. Molal depression constant \(K_f\) of benzene is \(4.9\text{ K kg mol}^{-1}\). What is the percentage association of the acid?

Read the following passage carefully and answer the given questions.
Insulin consists of two short polypeptide chains- chain A and chain B, that are linked together by disulphide bridges. Some strains of Bacillus thuringiensis produce proteins that kill certain insects such as lepidopterans, coleopterans and dipterans. Bt toxin protein is converted into an active form of toxin due to the alkaline pH of the gut which solubilise the crystals. Transgenic animals that produce useful biological products can be created by the introduction of the portion of DNA (or genes) which codes for a particular product such as human protein (alpha-1-antitrypsin) used to treat emphysema. Plants, bacteria, fungi and animals whose genes have been altered by manipulation are called Genetically Modified Organisms (GMO). GM plants have been useful in many ways. Genetic modification has: (i) made crops more tolerant to abiotic stresses. (ii) reduced reliance on chemical pesticides (pest-resistant crops). (iii) helped to reduce post harvest losses. (iv) increased efficiency of mineral usage by plants (this prevents early exhaustion of fertility of soil). (v) enhanced nutritional value of food.
Which one of the following species is not killed by insecticidal protein produced by Bacillus thuringiensis?

In gamma ray emission from a nucleus.

A square of side L meters lies in the xy-plane in a region where the magnetic field is given by \( \vec{B} = B_0(2\hat{i} + 4\hat{j} + 5\hat{k}) \, T \), where \( B_0 \) is constant. The magnitude of flux passing through the square is

Ultra pure silicon can be obtained by