CUET · BIOLOGY · PYQ PAPER 2025
Read the following paragraph carefully and answer the given questions.
In the year 1963 the two enzymes responsible for restricting the growth of bacteriophage in E. coli were isolated. The first restriction endonuclease - Hind II, whose functioning depended on a specific DNA nucleotide sequence was characterized five years later. It was found that Hind II always cut DNA molecules at a particular point by recognizing a specific sequence of six base pairs. This specific base sequence is known as the recognition sequence for Hind II. Besides Hind II, today we know more than 900 restriction enzymes that have been isolated from over 230 strains of bacteria, each of which recognize different recognition sequences.
When DNA from different sources is cut by the same restriction enzymes, the resultant DNA fragments have the :
- A Same kind of sticky- ends
- B Different kind of sticky- ends
- C Sticky ends with adenine and thymine only
- D Sticky ends rich in guanine and cytosine only
Answer & Solution
Correct Answer
(A) Same kind of sticky- ends
Step-by-step Solution
Detailed explanation
Same kind of sticky- ends
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