Match List-l with List-II

List-l (Term)	List-II (Function)
(A) Nuclease	(I) Synthesizes DNA from RNA
(B) Ligase	(II) Have both directions exonuclease activity and synthesize new polynucleotide
(C) DNA Polymerase I	(III) Degrades nucleic acids by breaking phosphodiester bonds
(D) Reverse Transcriptase	IV) Joins DNA molecules together by formation of phosphodiester bonds

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Read the paragraph given below and answer the question. Insulin used for diabetes was earlier extracted from slaughtered cattle and pigs. Insulin from an animal source, though caused some patients to develop allergy or other type of reactions. Insulin consists of two short polypeptide chains: chain A and chain B, that are linked bridges. In mammals, including human, insulin is synthesised as a pro-hormone. Patient with diabetes have:

Match List - I with List - II.

List - I	List - II
(A) Aspergillus niger	(I) Butyric acid
(B) Clostridium butylicum	(II) Acetic acid
(C) Saccharomyces cerevisiae	(III) Citric acid
(D) Acetobacter aceti	(IV) Ethanol

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Match List-l with List-ll

List-l (pollinations)	List-II (Features)
(A) Autogamy	(I) Flowers do not open
(B) Geitnogamy	(II) Transfer of pollen grains from anther to stigma of different plant
(C) Xenogamy	(III) Transfer of pollen grains from anther to stigma of another flower of same plant
(D) Cleistogamy	(IV) Transfer of pollen grains from anther to stigma of the same flower

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Read the following paragraph and answer the given questions:
In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes. Presence of more than one recognition site in the vector will generate several fragments, which will complicate the gene cloning. In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes. Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning. The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes. The recombinant plasmids will lose tetracycline resistance due to insertion of foreign DNA but can still be selected out from non-recombinant ones by plating the transformants on tetracycline containing medium.
The recombinants will grow in ampicillin containing medium but not on that containing tetracycline. But, non- recombinants will grow on the medium containing both the antibiotics. In this case, one antibiotic resistance gene helps in selecting the transformants, whereas the other antibiotic resistance gene gets 'inactivated due to insertion' of alien DNA, and helps in selection of recombinants. Origin of replication (ori) is a sequence from where replication starts and any piece of DNA when linked to this sequence can be made to replicate within the host cells. This sequence is also responsible for controlling the copy number of the linked DNA. So, if one wants to recover many copies of the target DNA it should be cloned in a vector whose origin support has high copy number.
In pBR322 'rop' codes for -

Which of the following is not a venereal disease?

Foetal ejection reflex triggers the release of:

If the matrix \(\left[\begin{array}{ccc}3 & 2 a & -5 \\ -4 & 0 & b \\ -5 & 3 & 7\end{array}\right]\) is symmetric, then the value of \((a+b)\) is :

Amniocentesis should not be used for:

Read the passage carefully and answer the questions
Nearly all the transition elements display typical metallic properties such as high tensile strength, ductility, malleability, high thermal and electrical conductivity and metallic lustre. The transition metals (with the exception of Zn, Cd and Hg) are very hard and have low volatility. Their melting and boiling points are high. They have high enthalpies of atomization. lons of the same charge in a given series show progressive decrease in radius with increasing atomic number. However, the variation within a series is quite small. The filling of 4f before 5d orbital results in a regular decrease in atomic radii called Lanthanoid contraction which essentially compensates for the expected increase in atomic size with increasing atomic number. The net result of the lanthanoid contraction is that the second and the third d series exhibit similar radii (e.g., Zr 160 pm, Hf 159 pm) and have very similar physical and chemical properties. There is an increase in ionisation enthalpy along each series of the transition elements from left to right. The first ionisation enthalpy, in general, increases, but the magnitude of the increase in the second and third ionisation enthalpies for the successive elements, is much higher along a series. One of the notable features of a transition elements is the great variety of oxidation states these may show in their compounds.
Atomic number of Fe, Co, Ni and Cu are 26, 27, 28 and 29 respectively. Which among them is expected to possess the highest value for the second ionization enthalpy?

Which of the following techniques are used for early molecular diagnosis of disease?
(A) Urine analysis
(B) PCR
(C) ELISA
(D) RDT
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The coordination number \(( CN )\) and oxidation state \(( OS )\) of Co in \(\left[ Co \left( C _2 O _4\right)_3\right]^{3-}\) is. \(\_\_\_\_\)

Identify the electromagnetic waves as per their following use/ applications.
(A) \( \lambda_1 \) is used to produce heating effect
(B) \( \lambda_2 \) is used in satellite communication
(C) \( \lambda_3 \) is absorbed by the ozone layer
(D) \( \lambda_4 \) is used for studying crystal structure
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