Match List - I with List - II.

List - I	List - II
(A) Cloning vector	(I) Sea weeds
(B) β-galactosidase	(II) Selectable marker
(C) Agarose	(III) Ti-plasmid of Agrobacterium tumifaciens
(D) \(a m p^{ R }\) in pBR322	(IV) Chromogenic screening

Choose the correct answer from the options given below :

The spermatids are transformed into spermatozoa by the process called as

Chromosomes 'Z' and 'W' for sex determination are seen in :

Match List-l with List-II

List-l (Genetic disorder)	List-II (Characteristics)
(A) Haemophilia	(I) Mutation in HBB gene
(B) Thalassemia	(II) Sex-linked recessive disease
(C) Phenylketonuria	(III) Aneuploidy
(D) Klinefelter's syndrome	(IV) Mental retardation and reduced hair and skin pigmentation

Choose the correct answer from the options given below:

Which of the following is a chromosomal disorder?

Which of the following is/are NOT the way(s) to introduce alien DNA into the host cell?
(A) Using vectors like Agrobacterium tumifaciens
(B) Injecting directly into the nucleus of animal cell
(C) Using ethylene dibromide
(D) Using vectors like retroviruses
(E) Using biolistics or gene gun
Choose the correct answer from the options given below:

Read the following passage carefully.
Restriction enzyme
Restriction enzymes cut the strand of DNA a little away from the center of the palindrome sites, but between the same two bases on the opposite strands. This leaves single stranded portions at the ends. There are overhanging stretches called sticky ends on each strand. These are named so because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase. The word palindromes refer to group of letters that forms the same words when read both forward and backward. Restriction endonucleases are used in genetic engineering as molecular scissors.
Which one of the following option is correct about restriction enzyme?

The value of the constant \(\lambda\), so that the function given below is continuous at \(x=-1\) is :
\(f(x)=\left\{\begin{array}{ll}\frac{x^2-2 x-3}{x+1}, & x \neq-1 \\\lambda, & x=-1\end{array}\right.\)

Osmotic pressure measurements are less accurate and difficult to carry out. Therefore, this method is not useful for measuring molecular weights. But it is used to find out molecular weights of polymers eg. plastics, proteins and starch having many monomer units. Knowing the value of osmotic pressure of a given solution, molecular weight of the solute can be calculated using Van't Hoff equation.
A solution which shows deviations from Raoult's law is called:

Read the passage carefully and answer the Questions
Amines are classified as primary \(\left(1^{\circ}\right)\), secondary \(\left(2^{\circ}\right)\) and tertiary \(\left(3^{\circ}\right)\) depending upon the number of hydrogen atoms replaced by alkyl or aryl groups in ammonia molecules. Aliphatic amines are a stronger bases due to \(+I\) effect of an alkyl groups.
Intermolecular hydrogen bonding in primary and secondary amines affects the boiling points of amines. The intermolecular association is more in primary amines than in secondary amines as there are more number of hydrogen atoms available for hydrogen bond formation. Nitro compounds are reduced to amines by passing hydrogen gas in the presence of finely divided nickel, palladium or platinum and also by reduction with metals in an acidic medium. Gabriel synthesis is used for the preparation of primary amines. Primary amines react with nitrous acid to give unstable diazonium-salts which can be used to synthesize a variety of compounds.
Identify the amine possessing least basic strength

A system of three charges -q, + Q and -q are placed at three points at equal distances on a straight line in the same order. If the potential energy of the system is found to be zero, find the ratio Q/q.

Two inductor coils of self inductance \( L_1 \) and \( L_2 \) having same length and same area of cross-section are placed so close that effective flux in one coil is completely linked with the other. The mutual inductance between them is, is equal to:

The area of the region enclosed between \(y^2=4 x\) and \(x=1\) and \(x=3\) in the first quadrant is: