Match List I with List II

LIST I	LIST II
A. One species is benefitted and other harmed.	I. Amensalism
B. One species is benefitted and other neither harmed nor benefitted.	II. Parasitism
C. Both the interacting species are benefitted.	III. Commensalism
D. One species is harmed and other remains unaffected.	IV. Mutualism

Read the following paragraph and answer the given questions.
RNAi takes place in all eukaryotic organisms as a method of cellular defense. The source of this complementary RNA could be from an infection by viruses having RNA genomes or mobile genetic elements (transposons) that replicate via an RNA intermediate. Insulin from an animal source, though caused some patients to develop allergy or other types of reactions to the foreign protein. Insulin consists of two short polypeptide chains: chain A and chain B, that are linked together by disulphide bridges. Transgenic mice are being developed for use in testing the safety of vaccines before they are used on humans. Transgenic mice are being used to test the safety of the polio vaccine. If successful and found to be reliable, they could replace the use of monkeys to test the safety of batches of the vaccine. Specific Bt toxin genes were isolated from Bacillusthuringiensis} and incorporated into the several crop plants such as cotton. The choice of genes depends upon the crop and the targeted pest, as most Bt toxins are insect-group specific. The toxin is coded by a gene cryIAc named cry. There are a number of them, for example, the proteins encoded by the genes cryIAc and cryIIAb control the cotton bollworms, that of cryIAb controls corn borer.

The suitable example of mobile genetic elements is:

Read the following passage carefully and answer the given questions.
Restriction endonuclease on finding its specific recognition sequence, bind to the DNA and cut each of the two strands of the double helix at specific points in their sugar -phosphate backbones. Each restriction endonuclease recognises a specific palindromic nucleotide sequences in the DNA. Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules such as RNA, proteins, polysaccharides and also lipids. This can be achieved by treating the bacterial cells/plant or animal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells), chitinase (fungus). Now a days the most commonly used matrix is agarose which is a natural polymer extracted from sea weeds. The DNA fragments separate (resolve) according to their size through sieving effect provided by the agarose gel. The first restriction endonuclease-Hind II, whose functioning depended on a specific DNA nucleotide sequence was isolated and characterised five years later. It was found that Hind II always cut DNA molecules at a particular point by recognising a specific sequence of six base pairs. In addition to 'ori', the vector requires a selectable marker, which helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants. Transformation is a procedure through which a piece of DNA is introduced in a host bacterium. Normally, the genes encoding resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, etc., are considered useful selectable markers for E. coli
Hind II always cut DNA molecules at a particular point by identifying a specific sequence of six base pairs. This specific base sequence is called _______ for Hind II.

Arrange the correct sequence of steps for gel electrophoresis.
(A). Load this DNA into the agarose gel matrix
(B). Cutting the DNA fragments by using a restriction enzyme
(C). Use ethidium bromide for DNA staining
(D). Separated bands of DNA are cut out from agarose gel
Choose the correct answer from the options given below.

Progestasert and LNG-20 are the examples of:

Which one of the following regions is not present in transcriptional unit of DNA

Which of the following is the highest species-rich taxonomic group among animals?

Daily life phenomena based on scattering of light are
(A) Blue colour of sky
(B) Clouds appearing white
(C) Twinkling of stars
(D) Danger signals being red
Choose the correct answer from the options given below:

In a step-down transformer, 22 kV is converted into 220 V. The number of turns in the primary coil is 60000. The number of turns in the secondary coil will be

Which of the following reaction will not produce ketones?

\(t_{99\%}\) with respect to \(t_{90\%}\) for a first order reaction is :

What is the structural formula for Freon-12.

The vector equation of the line passing through the point \((-1,5,4)\) and perpendicular to the plane \(z=0\) is :