Human insulin was prepared by:

Read the passage carefully and answer the given questions.
DNA Fingerprinting
Polymorphism in DNA sequence is the basis of genetic mapping of the human genome as well as of DNA fingerprinting. DNA fingerprinting involves identification of differences in some specific regions in the DNA sequence called repetitive DNA. It works on the principle of polymorphisms in DNA sequences. The repetitive DNA are separated from bulk genomic DNA. The bulk DNA forms a major peak and the other small peaks are referred to as satellite DNA. These repetitive/satellite DNA sequences show a high degree of polymorphisms and form the basis of DNA fingerprinting.
One of the most frequently used techniques in DNA fingerprinting is

Identify the correct statements with respect to microbes.
A. 'Toddy' the traditional drink obtained by fermenting sap of Palm tree.
B. Swiss cheese's large holes are due to activity of Saccharomyces cerevisae.
C. 'Penicillin' was discovered by Alexander Fleming.
D. The Cyclosporin A, is produced by Trichoderma polysporum.
Choose the the correct answer from the options given below:

Select the hormone which is not secreted by human placenta:

Plasmodium enters the human body as:-

DNA recombinant technology involves the use of restriction endonucleases. After having cloned the gene of interest and having optimised the conditions to induce the expression of the target protein, one has to consider producing it on a large scale. The cells harbouring cloned genes of interest may be grown on a small scale in the laboratory. In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes. Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning. In Biolistics or gene gun method cells are bombarded with high velocity micro particles of gold or tungsten coated with DNA. The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes. For example, you can ligate a foreign DNA at the BamH I site of tetracycline resistance gene in the vector pBR322. Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules such as RNA, proteins, polysaccharides and also lipids. The RNA can be removed by treatment with ribonuclease whereas proteins can be removed by treatment with protease. Other molecules can be removed by appropriate treatments and purified DNA ultimately precipitates out after the addition of chilled ethanol.
If any protein encoding gene is expressed in a heterologous host, it is called as

Observe the given figure and answer the following questions:

Match List - I with List - II.

List I	List ll
(A) Antigen binding site	(I) D
(B) Heavy chain	(II) A
(C) Light chain	(III) B
(D) C-Terminal	(IV) C

Choose the correct answer from the options given below:

The area of the region bounded by the curve \(x=y^2\), y-axis and the line y = 3 and y = 4 is:

Chronologically arrange the names of scientists who contributed to the study of genetics.
(A) Alfred Sturtevent
(B) Gregor Mendel
(C) Thomas Hunt Morgan
(D) Sutton and Boveri
Choose the correct answer from the options given below:

A source emits sound of frequency 600 Hz inside water. The frequency heard in air (velocity of sound in water is 1500 m/s and velocity of sound in air is 300 m/s) will be :

For an electric dipole in a non-uniform electric field with dipole moment parallel to the field, force F and torque t are :

If \( n_h \) is the number of holes per unit volume and \( n_e \) is the number of conduction electrons per unit volume in an extrinsic semiconductor. The relation between \( n_h, n_e \) and \( n_i \) is (\( n_i \) is intrinsic carrier concentration):

Which one of the following infections is completely curable?