CUET · BIOLOGY · PYQ PAPER 2025
Hind II is a restriction endonuclease which always cuts DNA molecule at a particular point by recognizing:
- A Specific promoter region of ten base pairs
- B Specific sequence of four base pairs
- C Specific sequence of six base pairs
- D Specific sequence of eight base pairs
Answer & Solution
Correct Answer
(C) Specific sequence of six base pairs
Step-by-step Solution
Detailed explanation
Specific sequence of six base pairs
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In the year 1963 the two enzymes responsible for restricting the growth of bacteriophage in E. coli were isolated. The first restriction endonuclease - Hind II, whose functioning depended on a specific DNA nucleotide sequence was characterized five years later. It was found that Hind II always cut DNA molecules at a particular point by recognizing a specific sequence of six base pairs. This specific base sequence is known as the recognition sequence for Hind II. Besides Hind II, today we know more than 900 restriction enzymes that have been isolated from over 230 strains of bacteria, each of which recognize different recognition sequences.
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