CUET · BIOLOGY · PYQ PAPER 2025
Entamoeba histolytica is a protozoan parasite found in which part of human body?
- A Lungs
- B Liver
- C Small intestine
- D Large intestine
Answer & Solution
Correct Answer
(D) Large intestine
Step-by-step Solution
Detailed explanation
Large intestine
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Some strains of `Bacillus thuringiensis` produce proteins that kill certain insects such as lepidopterans (tobacco budworm, armyworm), coleopterans (beetles) and dipterans (flies, mosquitoes). In `Bacillus thuringiensis` the toxin is coded by a gene `crylAc` named cry. There are a number of them, for example, the proteins encoded by the genes `crylAc` and `cryllAb` control the cotton bollworms, that of `crylAb` controls corn borer. Using conventional methods of diagnosis (serum and urine analysis, etc.) early detection is not possible. Recombinant DNA technology, Polymerase Chain Reaction (PCR) and Enzyme Linked Immuno-sorbent Assay (ELISA) are some of the techniques that serve the purpose of early diagnosis. The first clinical gene therapy was given in 1990 to a 4-year old girl with adenosine deaminase (ADA) deficiency. This enzyme is crucial for the immune system to function. The disorder is caused due to the deletion of the gene for adenosine deaminase. `RNAi` takes place in all eukaryotic organisms as a method of cellular defense. This method involves silencing of a specific mRNA due to a complementary `dsRNA` molecule that binds to and prevents translation of the `mRNA`.
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The DNA fragments can be separated by a technique known as gel electrophoresis. Since DNA fragments are negatively charged molecules, they can be separated by forcing them to move towards the anode under an electric field through a medium/matrix. The separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation. The repeated amplification of DNA is achieved by the use of a thermostable DNA polymerase (isolated from a bacterium, Thermus aquaticus). After completion of the biosynthetic stage, the product has to be subjected through a series of processes before it is ready for marketing as a finished product. The downstream processing and quality control testing vary from product to product. The convention for naming restriction enzymes is the first letter of the name comes from the genus and the second two letters come from the species of the prokaryotic cell from which they were isolated.
The thermostable DNA polymerase is isolated from -CUET 2025 Hard - Match List - I with List - II.
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When a host is exposed to antigens, which may be in the form of living or dead microbes or other proteins, antibodies are produced in the host body. This type of immunity is called active immunity. Active immunity is slow and takes time to give its full effective response. Injecting the microbes deliberately during immunisation or infectious organisms gaining access into body during natural infection induce active immunity. When antibodies are directly given to protect the body against foreign agents, it is called passive immunity.
Match List I with List IILIST I LIST II A. Physical Barrier I. Saliva B. Cellular Barrier II. Interferons C. Physiological Barrier III. Skin D. Cytokine Barrier IV. Polymorpho-nuclear leukocytes
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