CUET · BIOLOGY · PYQ PAPER 2025
DNA fingerprinting techniques have the following steps. Arrange these
steps in the correct order.
(A) Digestion by restriction enzyme
(B) Transferring separated DNA fragments
(C) DNA-DNA hybridization
(D) Gel Electrophoresis
Choose the correct answer from the options given below:
- A (A), (D), (B), (C)
- B (A), (B), (C), (D)
- C (Β), (A), (D), (C)
- D (C), (B), (D), (A)
Answer & Solution
Correct Answer
(A) (A), (D), (B), (C)
Step-by-step Solution
Detailed explanation
(A), (D), (B), (C)
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The separated DNA fragments can be visualized only after staining the DNA with ethidium bromide followed by exposure to UV radiation. Bright orange colored bands of DNA in ethidium bromide stained gel exposed to UV light can be seen. The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution. The DNA fragments purified in this way are used in constructing recombinant DNA by joining them with a cloning vector.
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