Arrange the given steps involved in Polymerase Chain Reaction in correct sequence?
(A) Annealing
(B) Extension
(C) Denaturation
(D) Amplification
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Read the given passage and answer the following questions:
Secondary treatment or biological treatment: The primary effluent is passed into large tanks where air is pumped into it. This allows vigorous growth of aerobic microbes. While growing, these microbes consume the major part of the organic matter in the effluent. This significantly change the BOD (biochemical oxygen demand) of the effluent. The effluent in then passed into a tank a small part of the sludge is pumped back into the aeration tank to serve as the inoculum. The remaining major part of the sludge is pumped into large tanks called sludge digesters. Here, other kinds of bacteria grow anaerobically in the sludge. The biogas produced can be used source of energy as it is inflammable. The effluent from the secondary treatment plant is generally released into natural water bodies like rivers and streams.
The anaerobic sludge digester

Arrange the following stages of primary succession in water in correct sequence.
A. Rooted submerged plants
B. Marsh meadows
C. Phytoplanktons
D. Scrubs followed by trees
E. Free floating plants
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Which one of the following statements is not correct regarding the DNA?

Genetically modified crops have advantages over normal crops as they are:
(A) More tolerant to cold, drought, salt and heat
(B) Less efficient in mineral usage
(C) Less reliant on chemical pesticides
(D) Nutritionally enriched
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Match List - l with List - ll

List - I (Terms)	List - II (Features)
(A) Allele	(I) The position on the chromosome where a gene occurs
(B) Locus	(II) Testing a suspected heterozygote by crossing it with a known homozygous recessive
(C) Gene	(III) One of two or more alternative nucleotide sequences at a single gene locus
(D) Test Cross	(IV) A length of DNA that codes for a particular polypeptide or protein

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MTP is considered safe up to ___________ weeks of pregnancy.

\(\alpha\)-D(+) Glucose and \(\beta\)-D(+) Glucose are

If y = - 4 a root of \(\left|\begin{array}{lll}y & 2 & 3 \\ 1 & y & 1 \\ 3 & 2 & y\end{array}\right|=0\), then the product of the other two roots is

The momentum of a photon associated with a radiation of frequency \(2 \times 10^{13} Hg\) is (Given: \(h=6.6 \times 10^{-34} J\) s and \(c=3 \times 10^8 m / s\) )

The following reaction is given :
\[CH_3-CH(Br)-CH(Br)-CH_3 \xrightarrow[\Delta]{2 NaNH_2} A\]
Identify \(A\) from the following:

What is the oxidation number of the metal of \([Co(H_2O)(CN)(ox)_2]^{2-}\) coordination entity?

DNA recombinant technology involves the use of restriction endonucleases. After having cloned the gene of interest and having optimised the conditions to induce the expression of the target protein, one has to consider producing it on a large scale. The cells harbouring cloned genes of interest may be grown on a small scale in the laboratory. In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes. Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning. In Biolistics or gene gun method cells are bombarded with high velocity micro particles of gold or tungsten coated with DNA. The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes. For example, you can ligate a foreign DNA at the BamH I site of tetracycline resistance gene in the vector pBR322. Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules such as RNA, proteins, polysaccharides and also lipids. The RNA can be removed by treatment with ribonuclease whereas proteins can be removed by treatment with protease. Other molecules can be removed by appropriate treatments and purified DNA ultimately precipitates out after the addition of chilled ethanol.
If any protein encoding gene is expressed in a heterologous host, it is called as