Arrange the following steps of Polymerase Chain Reaction in correct sequence
A. Denaturation of ds DNA
B. Extension of Primers
C. Amplification of desired DNA
D. Primer Annealing
Choose the correct answer from the options given below:

Read the passage carefully and answer the given questions.
Recombinant DNA Technology
Asexual reproduction preserves the genetic information, while sexual reproduction permits variation. Traditional hybridization procedures used in plants and animals breeding very often lead to inclusion and multiplication of undesirable genes along with the desired genes. The techniques of genetic engineering, which include creation of recombinant DNA, use of gene cloning and gene transfer, overcome this limitation and allow us to isolate and introduce only one or a set of desirable genes without introducing genes into the target organism.
In Recombinant DNA Technology, what is the role of the "selectable marker gene"?

Match List-l with List-II

List-l (Geological periods)	List-ll (Plant forms)
(A) Carboniferous	(I) Progymnosperms
(B) Cretaceous	(II) Monocotyledons
(C) Tertiary	(III) Gnetales
(D) Devonian	(IV) Seed ferns

Choose the correct answer from the options given below:

Identify the species which is not invasive:

Read the passage and answer the question : There are thousands of varieties of rice in India alone. The diversity of rice in India is one of the richest in the world. Basmati rice is distinct for its unique aroma and flavour and 27 documented varieties of Basmati are grown in India. There is reference to Basmati in ancient texts, folklore and poetry, as it has been grown for centuries. Several attempts have also been made to patent uses, products and processes based on Indian traditional herbal medicine, e.g. turmeric, neem. There has been growing realisation of the injustice, inadequate compensation and benefit sharing between developed and developing countries. The Indian Parliament has recently cleared a Bill that takes such issues into consideration, including patent terms emergency provisions and research and development initiative.
Identify the statements explaining biopracy
(A) Unauthorised exploitation of bio-resoures of a country
(B) Adequate compensation is given to one country from where bioresource is taken
(C) Exploitation of traditional knowledge to develop modern applications
(D) Equal benefit sharing between developed and developing nations
Choose the correct answer from the options given below:

Read the following passage carefully.
Restriction enzyme
Restriction enzymes cut the strand of DNA a little away from the center of the palindrome sites, but between the same two bases on the opposite strands. This leaves single stranded portions at the ends. There are overhanging stretches called sticky ends on each strand. These are named so because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase. The word palindromes refer to group of letters that forms the same words when read both forward and backward. Restriction endonucleases are used in genetic engineering as molecular scissors.
Which bond forms on sticky end of each strand?

If last year, there were 80 lotus plants in a lake. Then, 16 new lotus plants were added through reproduction and the current population is 96 now. The birth rate of lotus plant is

\(\int \frac{e^x(1+x) d x}{\cos ^2\left(e^x x\right)}\) is equal to:

If \(\left|\begin{array}{cc}3 x & 4 \\ 7 & x\end{array}\right|=\left|\begin{array}{ll}6 & 3 \\ 2 & 1\end{array}\right|\) then :

Answer the question on the basis of passage given below :
Aldehydes and ketones represent two essential classes of organic compounds
which are extensively used in the synthesis of a wide variety of other organic molecules.
Due to the inherent polarity of the central carbonyl group, these substances readily undergo nucleophilic addition reactions.
Broadly speaking, these addition reactions can be categorized into two types:
one in which simple addition of the nucleophilic reagent occurs across the \(> C = O\) bond, and the second type where the initial addition is subsequently followed by the elimination of a water molecule.
A variety of ammonia derivatives, including hydroxylamine, hydrazine, phenylhydrazine,
2, 4-dinitroplhenylhdrazine, and semicarbagide, all belong to this second category of reactions.
These specific derivatives are fundamentally used for the identification and definitive characterization of aldehydes and ketones. Significantly, both types of nucleophilic addition reactions are strongly influenced by both steric and electronic factors.
As a general rule, aliphatic aldehydes exhibit higher reactivity compared to aromatic aldehydes.
The aliphatic aldehydes demonstrate the ability to reduce both Fehling's solution and Tollen's reagent, whereas aromatic aldehydes are limited to reducing only Tollen's reagent.
In a similar manner to aldehydes, aromatic ketones are observed to be less reactive than aliphatic ketones towards all nucleophilic addition reactions.
In a separate context, phenols and carboxylic acids both exhibit acidic properties in nature.
Both classes of compounds readily dissolve in NaOH solution and cause blue litmus to turn red.
However, carboxylic acids stand out as much stronger acids than phenols and thus can decompose sodium bicarbonate \(\left( NaHCO _3\right)\) with the distinct evolution of \(CO _2\) gas, a reaction which phenols do not undergo.
It is important to note that both electron- donating and electron- withdrawing substituents significantly influence the acid strength of both aliphatic as well as aromatic carboxylic acids.
Furthermore, unlike all other common aliphatic acids, formic acid uniquely possesses reducing properties and simultaneously does not display the typical reactions characteristic of the alkyl group.
Fehling Solution A and B respectively, are :

The IUPAC name of the following compound is

Which of the following is not a natural method of birth control?

An electron accelerated with potential V has a de-Broglie wavelength \( \lambda \) associated with it. If the potential is changed to 4 V, the wavelength becomes