Arrange the following steps of genetically engineered insulin production.
(A) Isolation and purification of the insulin gene from beta cells of the
pancreatic islets
(B) Expression of gene in host cell
(C) Joining the two polypeptides by disulfide bonds
(D) Cloning of insulin gene in Escherichia coli cloning host cell
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Select the steps of PCR technique from the following: DU Buddy
A. Primer synthesis
B. Primer annealing
C. DNA denaturation
D. Primer extension
E. DNA cleavage
Choose the correct answer from the options given below:

Match List-l with List-ll

List-l (Genetic disorder)	List-II (Cause)
(A) Thalassemia	(I) 45+XΟ
(B) Sickle-cell anemia	(II) Formation of abnormal hemoglobin
(C) Klinefelter's syndrome	(III) Substitution of glutamic acid by valine
(D) Turner's syndrome	(IV) 47, XXY

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The cotyledon in the seed of grass family is called :

Read the following paragraph carefully and answer the given questions.
In the year 1963 the two enzymes responsible for restricting the growth of bacteriophage in E. coli were isolated. The first restriction endonuclease - Hind II, whose functioning depended on a specific DNA nucleotide sequence was characterized five years later. It was found that Hind II always cut DNA molecules at a particular point by recognizing a specific sequence of six base pairs. This specific base sequence is known as the recognition sequence for Hind II. Besides Hind II, today we know more than 900 restriction enzymes that have been isolated from over 230 strains of bacteria, each of which recognize different recognition sequences.
DNA fragments can be separated by a technique known as:

AIDS is a sexually transmitted disease (STD). Find out the correct
statement with reference to it
(A) It is caused by HIV belonging to the retrovirus group.
(B) It can pass through the placenta from an infected mother to her child.
(C) It can be diagnosed using ELISA.
(D) HIV enters into the B-lymphocytes and reproduces to produce progeny
of virus.
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Which one of the following is not the invasive alien species?

Which one of the following set of constraints does the given shaded region represent?

Arrange the principal values of the following functions in ascending order
(A) \(\operatorname{cosec}^{-1}(2)\)
(B) \(\tan ^{-1}(-\sqrt{3})\)
(C) \(\tan ^{-1}(1)\)
(D) \(\tan ^{-1}\left(\cos \frac{3 \pi}{2}\right)\)
Choose the correct answer from the options given below:

Consider the curves, \(x^2+y^2=1\) and \((x-1)^2+y^2=1\).
Area enclosed by the two curves is :

Area of the triangle ABC with vertices \(\left(a, a^2\right),\left(b, b^2\right)\) and \(\left(c, c^2\right)\) is equal to :

Which of the following statements is/are correct?
(A) \(|x|\) is continuous everywhere in its domain.
\((B)|x|\) is differentiable everywhere in its domain.
(C) \([x]\) is continuous at every integral point.
\((D)[x]\) is discontinuous at every integral point.
Choose the correct answer from the options given below :

Let \(R\) be the set of all real numbers and \(f: R \rightarrow\) Range \(f\) be given by \(f(x)=3 x^2+1\). Then \(f^{-1}\{1,2\}\) is :