Arrange the following steps involved in Polymerase Chain Reaction in correct sequence.
A. Denaturation of dsDNA by heating.
B. Extension of Primer
C. Annealing of Primer
D. Amplification of DNA
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The rate of biomass production is called productivity. It is expressed in terms of _______ to compare the productivity of different ecosystems.

Identify the correct statements for adenosine deaminase (ADA) deficiency.
(A) It is caused by the failure of synthesis of an enzyme, adenosine
deaminase.
(B) It leads to inherited immunodeficiency disorder, severe combined
immunodeficiency (SCID).
(C) It is attributed to a mutant ADA gene.
(D) Its treatment can be done by introducing ADA cDNA into the
hepatocytes.
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Match List I with List II.

LIST I	LIST II
A. Lactic acid bacteria	I. Saccharomyces cerevisiae
B. Ethanol	II. Propionibacterium sharmanii
C. Swiss Cheese	III. Lactobacillus
D. Penicillin	IV. Penicillium notatum

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Which of the following is used to make the bacterial cell as 'Competent cell' to take up DNA?

Read the passage carefully and answer the given questions.
Recombinant DNA Technology
Asexual reproduction preserves the genetic information, while sexual reproduction permits variation. Traditional hybridization procedures used in plants and animals breeding very often lead to inclusion and multiplication of undesirable genes along with the desired genes. The techniques of genetic engineering, which include creation of recombinant DNA, use of gene cloning and gene transfer, overcome this limitation and allow us to isolate and introduce only one or a set of desirable genes without introducing genes into the target organism.
In Recombinant DNA Technology, what is the role of the "selectable marker gene"?

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Immune System in the Body
The human immune system consists of lymphoid organs, tissues, cells and soluble molecules like antibodies. The primary lymphoid organs are bone marrow and thymus where immature lymphocytes differentiate into antigen-sensitive lymphocytes. After maturation the lymphocytes migrate to secondary lymphoid organs like spleen, lymph nodes, tonsils, Peyer's patches of small intestine and appendix. The secondary lymphoid organs provide the sites for interaction of lymphocytes with the antigen, which then proliferate to become effector cells. The bone marrow is the main lymphoid organ where all blood cells including lymphocytes are produced. Each antibody molecule has four peptide chains, two small called light chains and two longer called heavy chains. Hence, an antibody is represented as H2L2. Different types of antibodies are produced in our body. IgA, IgM, IgE, IgG are some of them. Techniques like radiography (use of X-rays), CT (computed tomography) and MRI (magnetic resonance imaging) are very useful to detect cancers of the internal organs.
The site of interaction of lymphocytes with antigen is

Match List-I (Name of the Base) with List-II (Structure)

List - I (Name of the Base)	List - II (Structure)
(A). Adenine (A)	(I)
(B). Cytosine (C)	(II)
(C). Thymine (T)	(III)
(D). Guanine (G)	(IV)

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Match List-I with List-II

List-I (Complex ion)	List-II (Hybridisation)
(A) \(\left[ CoF _6\right]^{3-}\)	(I) \(d^2 s p^3\)
(B) \(\left[ Co ( CN )_4\right]^{2-}\)	(II) \(s p^3 d^2\)
(C) \(\left[ FeCl _4\right]^{-}\)	(III) \(s p^3\)
(D)\(\left[ Co \left( C _2 O _4\right)_3\right]^{3-}\)	(IV) \(d s p^2\)

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Match List I with List - II.

List - I	List - II
(A) Produces crystals of toxic insecticidal proteins	(I) Meloidegyne incognitia
(B) Nematode which infects the roots of tobacco plant	(II) Agrobacterium tumefaciens
(C) Vector used to transfer the nematode specific gene into the host plant was obtained from	(III) Bacillus thuringiensis
(D) Plasmids used to produce the insulin chains were obtained from	(IV) Propionibacterium sharmanii
	(V) Eshcherichia coli

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The rate of change of volume of a sphere with respect to its surface area, when the radius is 6 cm is:

Which among the following are correctly matched?
A. \(C_6H_5OCH_2CH_3\) - Phenetole
B. \(C_6H_5O(CH_2)_6CH_3\) - Heptyl phenyl ether
C. \(C_6H_5OCH_3\) - Dimethyl ether
D. \(C_2H_5OCH_2CH_3\) - Methyl ethyl ether
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A small piece of metal wire is dragged in the region of magnetic flux \( 8 \times 10^{-4} \, \text{Wb} \) in 0.5 s. The length of the wire is perpendicular to the magnetic field. The emf induced in the wire would be: