Arrange the following events in correct sequence.
A. Formation of zygote
B. Formation of blastocyst
C. Implantation
D. Formation of morula
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Which of the following bond in DNA strand is broken by the action of restriction enzyme?

Read the following paragraph and answer the given questions:
DRUGS AND ALCOHOL ABUSE
The drugs, commonly abused are opioids, cannabinoids, and coca alkaloids. Opioids are the drugs, which bind to specific opioid receptors present in our central nervous system and gastrointestinal tract. Heroin, commonly called smack is chemically diacetylmorphine which is a white, odourless, bitter crystalline compound. Cannabinoids are a group of chemicals, which interact with cannabinoid receptors present principally in the brain. Natural cannabinoids are obtained from the inflorescences of the plant Cannabis sativa. The flower tops, leaves and the resin of cannabis plant are used in various combinations to produce marijuana, hashish, charas and ganja. Generally taken by inhalation and oral ingestion, these are known for their effects on cardiovascular system of the body. It interferes with the transport of the neuro-transmitter dopamine. Cocaine, commonly called coke or crack is usually snorted. It has a potent stimulating action on central nervous system, producing a sense of euphoria and increased energy. Excessive dosage of cocaine causes hallucinations. Other well-known plants with hallucinogenic properties are Atropa belladona and Datura. These days cannabinoids are also being abused by some sportspersons. Drugs like barbiturates, amphetamines, benzodiazepines, and other similar drugs, that are normally used as medicines to help patients cope with mental illnesses like depression and insomnia, are often abused. Morphine is a very effective sedative and painkiller, and is very useful in patients who have undergone surgery. Several plants, fruits and seeds having hallucinogenic properties have been used for hundreds of years in folk-medicine, religious ceremonies and rituals all over the globe.
'Coca alkaloids' are obtained from the plant-

Identify the correct sequence involved in the formation of female gametophyte in angiosperms?
(A) 2-nucleated embryo sac
(B) Functional megaspore
(C) Female gametophyte
(D) 4-nucleated embryo sac
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The chromosomal disorders are caused due to absence, excess or abnormal arrangement of one or more chromosomes. Failure of segregation of chromatids during cell division results in the gain or loss of chromosome(s), called aneuploidy.
Which of the following statements are correct with respect to the humans suffering from the disorder?
(A) One in every 800 live births shows this karyotype
(B) The affected individual has 45 chromosomes.
(C) Non-disjunction of chromosomes at the time of gamete formation causes this disorder.
(D) In this disorder, individual has 45 autosomes
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The direction of transport of sperm in human is-
(A). Vas deferens
(B). Vasa efferentia
(C). Epididymis
(D). Ejaculatory duct
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There are two kinds of nucleases - exonucleases and endonucleases. Exonucleases remove nucleotides from the ends of the DNA whereas, endonucleases make cuts at specific positions within the DNA. The cutting of DNA by restriction endonucleases results in the fragments of DNA. These fragments can be separated by a technique known as gel electrophoresis. Since DNA fragments are negatively charged molecules they can be separated by forcing them to move towards the anode under an electric field through a medium/matrix. Small volume cultures cannot yield appreciable quantities of products. To produce in large quantities, the development of bioreactors, where large volumes (100 - 1000 litres) of culture can be processed, was required. Thus, bioreactors can be thought of as vessels in which raw materials are biologically converted into specific products, individual enzymes, etc., using microbial plant, animal or human cells. PCR stands for Polymerase Chain Reaction. In this reaction, multiple copies of the gene (or DNA) of interest are synthesised in vitro using two sets of primers (small chemically synthesised oligonucleotides that are complementary to the regions of DNA) and the enzyme DNA polymerase.
Identify the correct enzyme to release the DNA from the fungal cells.?

The cell \(Zn \left| Zn ^{2+}(1 M ) \| Cu ^{2+}(1 M )\right| Cu\) has \(E_{\text {cell }}^{\circ}=1.10 V\).
The cell is allowed to be completely discharged at 298 K .
The relative concentration of \(Zn ^{2+} / Cu ^{2+}\) is:

Which of the followings should be doped with pure silicon to get an n-type and p-type semiconductors respectively?

A 20 m long horizontal wire extends from North-East to South-West. It is falling down with a speed of \(4 \mathrm{~m} / \mathrm{s}\) horizontally. The horizontal components of the earth's magnetic field is \(0.4 \times 10^{-4}\) T. Find the value of induced current in the wire if the resistance of wire is \(2 \Omega\) is :

An electric dipole with dipole moment \( 3 \times 10^{-9} \, \text{C m} \) is aligned at \( 30^\circ \) with the direction of uniform electric field of magnitude \( 6 \times 10^4 \, \text{N C}^{-1} \). What will be the magnitude of torque?

Let \(X\) and \(Y\) be any two invertible square matrices of order \(n\). Then which of the statements are true?
(A) \((A B)^{-1}=A^{-1} B^{-1}\)
(B) \((A B)^{-1}=B^{-1} A^{-1}\)
(C) \((A B)^T=A^T B^T\), where \(A^T\) denotes transpose of A
(D) \((A B)^T=B^T A^T\), where \(A^T\) denotes transpose of A
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ELISA is based on the principles of: