Answer the following questions feom the diagram given below :-

The samples in different lanes are from different sources but treated with a common restriction endonuclease. The maximum number of restriction sites are present in

Which of the following hormone is/are secreted by corpus luteum?
(A) Progesterone
(B) LH
(C) FSH
(D) Estrogen

You must have seen the weed Calotropis growing in abandoned fields. The plant produces highly poisonous _______ and that is why you never see any cattle or goats browsing on this plant.

Which one of the following statements is not true with reference to AIDS infection in human beings?

Read the given paragraph carefully and answer the following questions:
Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules such as RNA, proteins, polysaccharides and also lipids. This can be achieved by treating the bacterial cells/plant or animal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells), chitinase (fungus). There are some basic steps in genetically modifying an organism (i) identification of DNA with desirable genes; (ii) introduction of the identified DNA into the host; (iii) maintenance of introduced DNA in the host and transfer of the DNA to its progeny. Agrobacterium tumifaciens, a pathogen of several dicot plants is able to deliver a piece of DNA known as 'T-DNA' to transform normal plant cells into a tumor and direct these tumor cells to produce the chemicals required by the pathogen. Other molecules can be removed by appropriate treatments and purified DNA ultimately precipitates out after the addition of chilled ethanol. Selection of recombinants due to inactivation of antibiotics is a cumbersome procedure because it requires simultaneous plating on two plates having different antibiotics. Therefore, alternative selectable markers have been developed which differentiate recombinants from non-recombinants on the basis of their ability to produce colour in the presence of a chromogenic substrate. In this, a recombinant DNA is inserted within the coding sequence of an enzyme, ß'-galactosidase. This results into inactivation of the gene for synthesis of this enzyme, which is referred to as insertional inactivation.
Agrobacterium tumefaciens, a pathogen of several dicot plants, is able to deliver a piece of DNA known as:

Which one of the following infections is completely curable?

Read the following passage carefully.
Restriction enzyme
Restriction enzymes cut the strand of DNA a little away from the center of the palindrome sites, but between the same two bases on the opposite strands. This leaves single stranded portions at the ends. There are overhanging stretches called sticky ends on each strand. These are named so because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase. The word palindromes refer to group of letters that forms the same words when read both forward and backward. Restriction endonucleases are used in genetic engineering as molecular scissors.
Which one of the following enzyme helps to join DNA?

Match List I with List II

LIST I	LIST II
A. Pain killers	I. Antipyretics
B. Treatment of infections	II. Analgesics
C. Reducing fever	III. Antibiotics
D. Treatment of depression	IV. Tranquilizers

Choose the correct answer from the options given below:

Determine the value of k for which the function \(f(x)=\left\{\begin{array}{ll}\frac{x^2-9}{x-3}, & x \neq 3 \\ k, & x=3\end{array}\right.\) is continuous at x = 3

The correct order of increasing reactivity of C-X bond towards nucleophilic substitution reaction (SN1) in the following compounds is
(A) Chlorobenzene
(B) Benzyl Chloride
(C) Tertiary Butyl Chloride
(D) 1-Chlorobutane
Choose the correct answer from the options given below :

Monochlorination of toluene in sunlight followed by hydrolysis with aqueous \(NaOH\) yields :

DNA recombinant technology involves the use of restriction endonucleases. After having cloned the gene of interest and having optimised the conditions to induce the expression of the target protein, one has to consider producing it on a large scale. The cells harbouring cloned genes of interest may be grown on a small scale in the laboratory. In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes. Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning. In Biolistics or gene gun method cells are bombarded with high velocity micro particles of gold or tungsten coated with DNA. The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes. For example, you can ligate a foreign DNA at the BamH I site of tetracycline resistance gene in the vector pBR322. Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules such as RNA, proteins, polysaccharides and also lipids. The RNA can be removed by treatment with ribonuclease whereas proteins can be removed by treatment with protease. Other molecules can be removed by appropriate treatments and purified DNA ultimately precipitates out after the addition of chilled ethanol.
Which one of the following enzymes works as molecular glue?

A solenoid with an iron core and a bulb are connected to an a.c. source. The bulb is glowing with certain brightness. When the iron core is removed from the solenoid, the brightness of the bulb